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Chemical Biology · LearnSpace
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Chemical Biology

Курс от University of Geneva
Средний≈ 21.1 чАнглийский
О курсеНавыкиПрограммаПреподаватели

О курсе

Chemical biology is a burgeoning field that has rapidly risen to prominence. This surge of interest has been fuelled by chemical biology’s applicability to understanding critical processes in live cells or model organisms in real time. This success has arisen because chemical biology straddles a nexus between chemistry, biology, and physics. Thus, chemical biology can harness rapid chemistry to observe or perturb biological processes, that are in turn reported using physical assays, all in an otherwise unperturbed living entity. Although its boundaries are endless, the multidisciplinary nature of chemical biology can make the field seem daunting; we beg to differ! Here, we deconstruct chemical biology into its core components, and repackage the material. In the process we build up for each student a practical and theoretical knowledge bank that will set these students on their way to understanding and designing their own chemical biology experiments. We will discuss fluorescence as a general language used to read out biological phenomena as diverse as protein localization, membrane tension, surface phenomena, and enzyme activity. We will proceed to discuss protein labeling strategies and fusion protein design. Then we will discuss larger and larger scale chemical biology mechanism and screening efforts. Highlights include a large amount of new data, tailored in the lab videos, and a large number of skilled presenters.

Навыки, которые вы освоите

BiochemistryCell BiologyMolecular BiologyBiochemical AssaysBiotechnologyLaboratory ResearchBiological EngineeringChemistryAnalytical TestingDrug DevelopmentBiologySpecimen LabelingScientific Visualization

Программа курса

6 модулей · 106 учебных материалов

01Welcome to Chemical Biology: Concepts and techniques20 материалов

The boundless trajectories of chemical biology

TrailerВидеоWelcomeВидеоCourse AcknowledgementsЧтениеWhy study chemical biology?Видео

Учитесь у экспертов

Robbie Loewith

Prof. Dr.

Marcus J. C. Long

Dr

Chemical Biology
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Обучение на Coursera

≈ 21.1 ч

6 модулей

Язык: Английский

Субтитры: Арабский, Французский, Бенгальский, Украинский, Китайский (Китай), Греческий, Итальянский, Бразильский португальский, Нидерландский, Корейский, Немецкий, Пушту, Урду, Русский, Тайский, Индонезийский, Шведский, Турецкий, Азербайджанский, Испанский, Дари, Хинди, Японский, Казахский, Венгерский, Польский

Часть программы вашего университета
Course outline and highlightsВидео
📢 Important preliminary information: Evaluations and subtitlesЧтение

Chemical Biology - Concepts 1

The foundation: the cellЧтениеComponents of a cellЗаданиеThe workhorses: proteins, enzymesЧтениеThe building blocks: monomeric units of proteinsВидео

Chemical Biology - Concepts 2

The building blocks: monomeric units of DNA and membranesВидеоAmino acidsЗаданиеMessengers and messages: posttranslational modifications, and pathwaysВидео

An eye opener; visualizing biomolecules

Classical protein readoutsЧтениеFlash mob: overview of protein analysis methodsЧтениеFlash mob - ChemistryЧтениеStudying cellular processes with chemical biology in real timeВидеоMatch technique to goalЗадание

Quiz time

📢 Important preliminary information: Evaluations and subtitlesЧтениеFinal Quiz Number 1Задание
02The Devil is in the Minutest Detail13 материалов

Traditional fluorescence microscopy – getting physical

Welcome to Module 2ВидеоPhysics of fluorescenceЧтениеLight'em up! Fluorophores: structures, and fluorescent principlesВидеоGet out (almost) what you put in!ВидеоJablonski diagramЗаданиеFluorescent proteinsЧтение

Traditional fluorescence microscopy – getting the picture?

RNA interference and genome editingЧтениеPlasmids and transfectionЧтениеDNA double stranded breaks and γ-H2AXЧтениеTraditional light microscopy – applicationsВидео

Traditional fluorescence microscopy – beyond seeing to believing

Traditional light microscopy – precautionsВидеоMatch color of fluorophore to structureЗадание

Quiz time

Final Quiz Number 2Задание
03A ruler over time and space! Fluorescent assays to measure complex parameters in real time23 материалов

Building modified proteins

Welcome to Module 3ВидеоDNA organization in eukaryotes is linked to regulationЧтениеCreating modified chromatin ВидеоKinetics of chemical reactionsЧтениеComparing chemical structuresЧтениеFlash mob inteinsЧтениеMaking proteins via expressed protein ligation and alkylationЗадание

TIRF talk: study of chromatin PTMs

PRC2, a DNA binding complex linked to development and cancerВидеоTIRF talkВидеоOn our TIRFВидеоIf your protein surfs on DNA, hit the TIRF to study itВидеоHit the tirf: applications of TIRF microscopyЗаданиеPHF1 Prolongs the stable PRC2-DNA complex, promoting activityВидео

Out with the old, in with the new, using fluorescence to measure membrane tension

Fluid mosaic modelВидеоDon’t get uptight! New methods to measure membrane tensionВидеоGetting a grip on membrane tension measurementsВидеоModern fluorescence methods, the spark of creativityЗаданиеModel OrganismsЧтение

A new twist on signaling regulation

TORCing TORC: the many faces of the TOR proteinsВидеоTORCing it out: pathways that seek to sense and restore membrane tensionВидеоTORCing about Tor's role in membrane tension regulationЗадание

Quiz time

Final Quiz 3Задание
04Putting proteins to work for us20 материалов

Getting tied down: using modified protein domains to localize pharmocophores in cells

Welcome to Module 4ВидеоMany enzyme mechanisms proceed through a covalent enzyme intermediate that can be stabilized through mutagenesisЧтениеTracking a specific protein in live cells & in real timeВидеоMolecular zipcodesВидео

Bringing it all together

How to design your fusion proteinВидеоOptimal considerations for making fusion proteins and employing them in chemical biologyЗаданиеOptimal considerations for making fusion proteins and employing them in chemical biology2Задание

Seeing what is right under your nose; measurement of metabolites using SNIFITs

Small molecule signalsЧтениеDesign principle and optimization of SNIFITsВидеоSNIFITs to ID neurotransmitters and drugsВидеоA bit on the nose? Choose the right SNIFITsЗадание

Sniffing out translational applications

Application to point of care diagnosisВидеоDesign your own SNIFITВнешний инструмент

Breaking free: untethered protein-specific modification methods with locale specificity

Biological electrophiles, the cell’s way of saying “I’m stressed”?ВидеоBreaking the tether is not that badВидеоT-REX: a question of control(s)ВидеоA perfect match?Внешний инструмент

Resolving the conflicts of reactive molecule signaling

T-REX rewiring of kinase activity through targeting AKT and PTEN: opposing forces meet ВидеоWeighing up our optionsЧтение

Quiz time

Final Quiz Number 4Задание
05Making light work of it on a larger and larger scale17 материалов

Lipid transport a slippery problem with a slick solution

Identify conflicting pathways regulated by lipid signalsВнешний инструментWelcome to Module 5ВидеоA fat lot of use? Proteins that bind and transport lipidsВидеоGreasing the wheels of discovery for new interactomesВидео

Photocaged strategies shed light on control of lipid metabolism at the subcellular level

SphingolipidsЧтениеLaser guided lipidsВидеоTo cage or not to cage?ЗаданиеDeuterium labeling to assess how sphingosine originating in lysosomes is metabolizedВидео

It takes two: deploying methods in tandem

Deuterium labeling and knockout combinedВидеоinterpretation of pulse chase data from wild type and knockdown linesЗаданиеMitochondria-released sphingosine does not elicit calcium signaling: global sphingosine release doesВидеоMatch molecular tag with the correct subcellular localeВнешний инструмент

Direct assays for electrophile sensing in cells or specific locales

Endogenous electrophile signalingЧтениеG-REX – a method to release specific electrophiles on demandВидеоVariables G-REX controls that traditional methods do notЗаданиеG-REX identifies important novel signaling proteins that function in fishВидео

Quiz time

Final Quiz Number 5Задание
06Casting wide your net13 материалов

Getting a grip: principals of barcoded library design

Welcome to Module 6ВидеоThe more, the merrier: Phage display, necessity, strengths and weaknessesЧтениеPeptide Nucleic Acid – Use in library synthesis and split and mix approachВидеоIn the lab - split and mix approach in a nutshellВидеоWorkflow for split and mixЗадание

PNAs in action

PNA libraries show their MITEВидеоIt takes two: library diversification through DNA displayВидеоDifferent methods to achieve diversityЗадание

A STING operation? Pathway specific screens

A STING operation: inhibiting a protein implicated in numerous inflammatory diseasesВидеоcomponents and controls for Luciferase assayЗаданиеPerforming the ScreenВидеоMutagenesis: ensuring we stay on targetВидео

Quiz time

Final Quiz Number 6Задание
Analyzing data from TIRF experiments to gain mechanistic insightsЗадание