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Getting started in cryo-EM · LearnSpace
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Getting started in cryo-EM

Курс от Caltech
Уровень не указан≈ 21.4 чАнглийский
О курсеНавыкиПрограммаПреподаватели

О курсе

This class covers the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. Philosophy: The course emphasizes concepts rather than mathematical details, taught through numerous drawings and example images. It is meant for anyone interested in the burgeoning fields of cryo-EM and 3-D EM, including cell biologists or molecular biologists without extensive training in mathematics or imaging physics and practicing electron microscopists who want to broaden their understanding of the field. The class is perfect as a primer for anyone who is about to be trained as a cryo-electron microscopist, or for anyone who needs an introduction to the field to be able to understand the literature or the talks and conversations they will hear at cryo-EM meetings. Pre-requisites: The recommended prerequisites are college-freshman-level math, physics, and biochemistry. Pace: There are 14.5 hours of lecture videos total separated into 40 individual “modules” lasting on average 20 minutes each. Each module has at the end a list of “concept check” questions you can use to test your knowledge of what was presented. As the modules are grouped into seven major subjects, one reasonable plan would be to go through one major subject each day. That would mean watching a couple hours of lecture and spending another hour or so thinking through the concept check questions each day for a week. Another reasonable plan would be to go through one module each day for a little over a month, or even three modules a week (Monday, Wednesday, and Friday) for a 3-month term. It is likely that as you then move on to actually begin using a cryo-EM or otherwise engage in the field, you will want to repeat certain modules.

Навыки, которые вы освоите

Digital Signal ProcessingImage QualityEngineering, Scientific, and Technical InstrumentsLaboratory TechniquesSpecimen PreparationLaboratory EquipmentMedical ImagingCell BiologySpatial AnalysisImage AnalysisScientific VisualizationBiomedical Technology

Программа курса

8 модулей · 90 учебных материалов

01Welcome6 материалов

Welcome

Introduction to the CourseВидеоWelcome from Professor JensenЧтениеAbout the InstructorЧтениеDescription of PartsВидео

Учитесь у экспертов

Grant J. Jensen

Professor of Biophysics and Biology; Investigator, Howard Hughes Medical Institute

Getting started in cryo-EM
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≈ 21.4 ч

8 модулей

Язык: Английский

Субтитры: Арабский, Французский, Бенгальский, Украинский, Китайский (Китай), Греческий, Итальянский, Бразильский португальский, Нидерландский, Корейский, Немецкий, Пушту, Урду, Русский, Тайский, Индонезийский, Шведский, Турецкий, Азербайджанский, Испанский, Дари, Хинди, Японский, Казахский, Венгерский, Польский

Часть программы вашего университета
Introduction: Why Electrons?Видео
Concept Check Questions: Why Electrons?Чтение
02Currents, Coils, Knobs and Names: Basic anatomy of the electron microscope17 материалов

Currents, Coils, Knobs and Names: Basic anatomy of the electron microscope

Introduction: Basic Anatomy of the EMВидеоElectron GunsВидеоConcept Check Questions: Electron GunsЧтениеEM LensesВидеоConcept Check Questions: Electron LensesЧтениеThe ColumnВидеоConcept Check Questions: The ColumnЧтениеSample ChamberВидеоConcept Check Questions: The Sample ChamberЧтениеEnergy FiltersВидеоConcept Check Questions: Energy FiltersЧтениеDetectorsВидеоConcept Check Questions: Electron DetectorsЧтениеVacuum SystemsВидеоConcept Check Questions: Vacuum SystemsЧтениеSummary and SafetyВидеоConcept Check Questions: Summary and SafetyЧтение
03Fourier Transforms and Reciprocal Space for Beginners13 материалов

Fourier Transforms and Reciprocal Space for Beginners

Introduction: Fourier Transforms for BeginnnersВидео1-D Sine Waves and their SumsВидеоConcept Check Questions: 1-D Sine Waves and Their SumsЧтение1-D Reciprocal SpaceВидеоConcept Check Questions: 1-D Reciprocal SpaceЧтение2-D Waves and ImagesВидеоConcept Check Questions: 2-D Waves and ImagesЧтение2-D Transforms and FiltersВидеоConcept Check Questions: 2-D Transforms and FiltersЧтение3-D Waves and TransformsВидеоConcept Check Questions: 3-D Waves and TransformsЧтениеConvolution and Cross-CorrelationВидеоConcept Check Questions: Convolution and Cross-correlationЧтение
04Image Formation13 материалов

Image Formation

Introduction: Image FormationВидеоAmplitude and Phase ContrastВидеоConcept Check Questions: Amplitude and Phase ContrastЧтениеWave Propagation and Phase ShiftsВидеоConcept Check Questions: Wave Propagation and Phase ShiftsЧтениеThe Contrast Transfer FunctionВидеоConcept Check Questions: The Contrast Transfer FunctionЧтениеDefocus and its EffectsВидеоConcept Check Questions: Defocus and its EffectsЧтениеEnvelopesВидеоConcept Check Questions: EnvelopesЧтениеCTF CorrectionВидеоConcept Check Questions: CTF CorrectionЧтение
05Fundamental Challenges in Biological EM11 материалов

Fundamental Challenges in Biological EM

Introduction: Fundamental Challenges in Biological EMВидеоSample Prep - Room Temperature MethodsВидеоConcept Check Questions: Sample Prep - Room Temperature MethodsЧтениеSample Prep Methods Involving FreezingВидеоConcept Check Questions: Sample Prep - Methods Involving FreezingЧтениеSample Prep GridsВидеоConcept Check Questions: Sample Prep - GridsЧтение3-D ReconstructionВидеоConcept Check Questions: 3-D ReconstructionЧтениеDose LimitationsВидеоConcept Check Questions: Dose LimitationsЧтение
06Single Particle Analysis11 материалов

Introduction to single particle analysis

Concept check questions: Introduction to single particle analysisЧтениеSpecial sample prep issuesВидеоConcept Check Questions: Special sample prep issuesЧтениеData collection (imaging)ВидеоConcept Check Questions: Data collection (imaging)ЧтениеReconstruction basicsВидеоConcept Check Questions: Reconstruction basicsЧтениеReconstruction: additional topicsВидеоConcept Check Questions: Reconstruction - additional topicsЧтениеInterpretation and limitationsВидеоConcept Check Questions: Interpretation and limitationsЧтение
07Tomography10 материалов

Tomography

Introduction: TomographyВидеоConcept Check Questions: Introduction: TomographyЧтениеTomography Data Collection and ReconstructionВидеоConcept Check Questions: Tomography Data Collection and ReconstructionЧтениеTomography Identifying Objects of InterestВидеоConcept Check Questions: Identifying Objects in TomogramsЧтениеTomography LimitationsВидеоConcept Check Questions: Tomography LimitationsЧтениеTomography Parameters and HandednessВидеоConcept Check Questions: Tomography Parameters and HandednessЧтение
082-D Crystallography9 материалов

2-D Crystallography

Introduction: 2-D Crystallography and Sample PrepВидеоConcept Check Questions: Introduction: 2-D Crystallography and Sample PrepЧтениеFT of a 2-D CrystalВидеоConcept Check Questions: Fourier Transform of a 2-D CrystalЧтение2-D Crystallography Data Collection and ReconstructionВидеоConcept Check Questions: Data Collection and ReconstructionЧтениеHelical TubesВидеоFarewellВидеоConcept Check Questions: Helical TubesЧтение