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What Are Viruses And How Do We Work With Them? · LearnSpace
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What Are Viruses And How Do We Work With Them?

Курс от Imperial College London
Средний≈ 11.7 чАнглийский
О курсеНавыкиПрограммаПреподаватели

О курсе

This course describes the basic nature if viruses and basic concepts of how they replicate. It looks at traditional methods for propagating and assaying viruses as well as methods to identify and follow them in individuals and populations. It will also look at how we can use molecular genetics to learn how viruses work in molecular detail. On successful completion of the course, learners will be able to: 1. Recognise unifying principles but diversity of virus structure and function 2. Illustrate how we can detect, identify and track viruses in individuals and populations 3. Recognise the importance of traditional and molecular genetics to understanding viruses This is an intermediate series of courses intended for both clinical and non-clinical scientists who want to update their skills for work in clinical, academic, and pharmaceutical/bioscience industries, and is developed and delivered by experts working on viruses and novel vaccines. To be successful in this series of courses, you should have basic knowledge in biology, genetics, microbiology or related fields.

Навыки, которые вы освоите

Molecular BiologyMicrobiologyBiotechnologyDiagnostic TestsCell CulturesEpidemiologyCell BiologyLaboratory TestingLife SciencesLaboratory ResearchInfectious DiseasesMolecular, Cellular, and MicrobiologyBiology

Программа курса

5 модулей · 66 учебных материалов

01Module 1 - What are viruses?13 материалов

Course introduction

Introduction to what are viruses and how do we work with them Видео

Prologue

Course syllabusЧтениеIntroduction to viruses and how we work with themЧтениеViruses and virus diversityВидео

Учитесь у экспертов

Mike Skinner

Emeritus Professor in Virology

What Are Viruses And How Do We Work With Them?
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Обучение на Coursera

≈ 11.7 ч

5 модулей

Язык: Английский

Часть программы вашего университета
Identify the type of pathogenЗадание

The central dogma of molecular biology

Introduction to the central dogma of molecular biologyЧтениеFrancis Crick’s “Central Dogma of Molecular Biology”, and virus modifications to itВидео

What are viruses?

Introduction to the virion and the viral genomeЧтениеWhat are viruses? The virionВидеоWhat are viruses? The viral genomeВидео

Summary of ‘what are viruses?’

Summary of ‘what are viruses?’ВидеоInternational Committee of the Taxonomy of Viruses (ICTV)ЧтениеSummative QuizЗадание
02Module 2 - How do viruses replicate (and spread)?15 материалов

Overview of virus replication

Overview of virus replicationЧтениеOverview of replicationВидео

Generic replication cycle

Introduction to the generic virus replication cycleЧтениеGeneric replication cycle, part 1 - receptor-mediated binding, fusion and entryВидеоMore on attachment and fusionЧтениеInterview - Receptor-mediated entry for influenzaВидеоInterview - Receptor-mediated entry for SARS-CoV-2ВидеоGeneric replication cycle, part 2 - intracellular transport, virus factories and shelterВидеоWhy do virions of this virus need to incorporate and deliver copies of their polymerase?Задание

Baltimore Group I, II and IV replication

Introduction to the Baltimore classificationЧтениеdsDNA & ssDNA (Baltimore Group I & II) expression and replication strategiesВидеоExpression and replication strategies – Baltimore Group IV (positive-sense ssRNA)Видео

Baltimore Group V, VI and VII replication

-ssRNA (Baltimore Group V) expression and replication strategiesВидеоReversivirus (Baltimore Group VI retroviruses & VII hepadnaviruses) replication strategiesВидеоVirus replication and spreadЗадание
03Module 3 - How do we propagate, observe and measure viruses?16 материалов

Overview of virus propagation and passage

Introduction to virus propagation and passageЧтениеOverview of virus propagation and passage Видео

Assays

Introduction to assaysЧтениеUsing serological assays in the home or in the workplaceЗаданиеInfectivity assays: dilution, plaques and TCID50ВидеоSerological assays: antigen and anti-viral antibodyВидеоViral genome and/or mRNA detectionВидеоInterview with Graham Taylor/Cooke about COVID lab diagnosticsВидеоWhich type of assay is best for …?Задание

Microscopy

Introduction to microscopyЧтениеMicroscopy – light and electron, classical and modernВидео

Determining virus structure

Introduction to determining virus structureЧтениеDetermining virus structure: crystallography and Cryo-EM Видео

Modern virus detection

Introduction to modern virus detectionЧтениеModern virus detection: virochips and deep sequencingВидеоPropagating, observing and measuring virusesЗадание
04Module 4 - How do viruses evolve?10 материалов

Virus mutation

Introduction to how viruses evolveЧтениеVirus mutation: quasispecies, phenotype, non-synonymous mutations, and variantsВидеоVirus mutation: indels, amplification, acquisition, and further evolutionВидеоVirus mutation: selection, reversion, fitness, and bottlenecksВидеоTypes and consequences of virus mutationЗадание

Vaccine reversion

Introduction to vaccine reversionЧтениеPoliovirus Sabin vaccine reversion – threat to eradication and possible solutionsВидеоUsing reverse genetics to eradicate polio?Обсуждение

Evolution of novel viruses

The evolution of novel viruses (reassortment, recombination, and adaptation)ВидеоHow viruses evolveЗадание
05Module 5 - How do we use ‘reverse genetics’ & ‘chimaeric viruses’ in research, gene therapy & vaccine development?12 материалов

The limitations of conventional virus genetics

Introduction to how we use reverse genetics and chimaeric virusesЧтениеThe limitations of conventional virus geneticsВидео

Recovery of viruses from cloned cDNA

Introduction to the recovery of viruses from cloned cDNAЧтениеWhat are the advantages of recovery of viruses from cloned cDNA?Видео

Reverse genetics

Introduction to reverse genetics and chimaeric virusesЧтениеWhat can we learn from ‘reverse genetics’?ВидеоHow can we use reverse genetics and chimaeric viruses?ВидеоConventional virus genetics, virus recovery and reverse geneticsЗаданиеHow do we balance the benefits & risks of ‘reverse genetics’?Видео

Conclusion

Gain-of-function researchОбсуждениеHow we use ‘reverse genetics’, ‘chimaeric viruses’ in research, gene therapy & vaccine developmentЗаданиеCourse SummaryВидео